cross-linked casein micelles hydrogel as carrier for ... · mv Ø ~ 160 nm ζ-potential 12.7 mv...
TRANSCRIPT
Cross-linked casein micelles hydrogel
as carrier for JABUTICABA (Myrciaria cauliflora)
polyphenolic extract
Luis Gustavo Lima Nascimento, Federico Casanova, Naaman Francisco Nogueira Silva,
Álvaro Viana Novaes de Carvalho Teixeira, Márcia Cristina Teixeira Ribeiro Vidigal, Paulo César Stringheta and
Antônio Fernandes de Carvalho
1
JABUTICABA
FOOD
SUPPLEMENTS
BODY CARE
Salomão et al. (2018)
2
SOURCE OF POLYPHENOLSSalomão et al. (2018)
8.5 % POLYPHENOLS
50% Fruit peel and seed
2.0 % ANTHOCYANINS
5.0 % seed ELLAGIC ACID
Chronic diseases
Polyphenols reduce the risk of disease
Cancer Cardiovascular diseases Diabetes 3
Rodrigues et al. (2015)
92.8 mg/g GALLIC ACID EQUIVALENT
7.8 mg/g ELLAGIC ACID
4.9 mg/g CYANIDIN-3-O-GLUCOSIDE
4.8 mg/g ANTHOCYANIN
POLYPHENOLS EXTRACTION
Ethanol Water Ultrasound
Peel
Roomtemperature
Unstable
• basic pH
• presence of light and oxygen
4
WHY CASEIN MICELLES ?
5
CMs
Tetra pak (1996); Sadat-Mekmene et al. (2011); He et al. (2016); Casanova et al. (2018)
WHY CASEIN MICELLES ?
Cyanidin-3-O-glucoside
Malvidin-3-O-glucoside
Hydrophobic interactionsANTHOCYANINS
CASEIN MICELLE (CMs)
αS- caseins β-casein
Hydrophilic interactions(van der Waals forces or hydrogen bonding)
6
45 °C
1 h
Nascimento et al, 2019
Cross-linked micelle (CMs)
CROSS-LINKED
CASEIN MICELLES
Transglutaminase(Tgase)
Jabuticaba extract
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Nascimento et al, 2019
Cross-linkedCMs
OBJECTIVE
GEL
Develop an acid hydrogel from cross-linked CMs capable of loading and
delivering anthocyanins extracted from jaboticaba peel
OR2% w.w-1
glucono – δ – lactone pH 4.5
Dynamic Light Scattering (DLS)
ζ-potential
SDS-PAGE
Dynamic rheological measurements
Water holding capacity
Confocal scanning laser microscopy 8
METHODS
IN SUSPENSION IN GEL
Ø ~177 nm
ζ-potential 12.7 mV
Ø ~160 nm
ζ-potential 12.7 mV
Size of CMs is not altered in the presence
of the extract
Ø ~1.6 µm Ø ~0.66 µm
Water rentation
55% higher
The extract altered the porous size
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CMS
CMS + Jaboticaba
CMS + Tgase
CMS + Tgase + Jaboticaba
Dynamic oscillatory rheology
(□) CMS without Tgase or JE
(○) CMS with Tgase
(■)CMS without Tgase and with JE
(●) CMS with Tgase and JE
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Shear stress sweep Frequency sweep
• Similar behavior of the hydrogel at the end of linear viscosity
• Different acidification time : samples with Jaboticaba extract (C and D) reaches pH 4.5 forty minutes before samples without Jaboticaba extract (A and B) (data not shown)
• Tgase increased hydrogel strength while Jaboticaba extract decrease it
0,1
1
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0,001 0,01 0,1 1 10 100 1000 104
loss ta
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t (
shear stress (Pa)
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0,35
0,4
0,45
0,5
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loss ta
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t (
frequency (Hz)
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0 100 200 300 400
%R
elea
se
Time (min)
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0 100 200 300 400
%R
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se
Time (min)
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0 100 200 300 400
%R
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Time (min)
Casein micelles
Crosslinked Casein micelles
pH 2.0
pH 4.5pH 7.0
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Release profile of
jaboticaba extract
CONCLUSION and PERSPECTIVES
In suspension, Jabuticaba extract did not change the
casein micelles properties
Gel formed by crosslinked CMs has smaller
porous and prolonged release of Jabuticaba
extract (10 % slower)
The hydrogels can be applied to carry,
delivery and controlled release Jabuticaba
extract from neutral to acidic pH
12
In vivo release properties of Jaboticaba extract
