umr cnrs 6022 génie enzymatique et cellulaire (directeur : a. friboulet) thème 1 : biocatalyse et...

UMR CNRS 6022Génie Enzymatique et Cellulaire

(Directeur : A. Friboulet)

Thème 1 : Biocatalyse et Alternatives Métaboliques (Prof. D. Thomas)

Thème 2 : Biocatalyse supramoléculaire et anisotrope

•Métabolisme lipidique végétal (Dr. B. Thomasset, Dr. Y. Perrin)

•Génèse et caractérisation de la diversité biocatalytique (Dr. A. Friboulet, Dr. B. Bihan-Avalle, Dr. S. Padiolleau, Dr C. Bedel)

•Polymères biomimétiques, impression moléculaire et nanostructuration (Pr. K. Haupt, Dr A. Cordin)

•RMN des solides et systèmes biomimétiques (Pr. C. Sarazin)

•Modèles biomimétiques membranaires (Pr. S. Pulvin, Dr. S. Morandat, Dr. V. Ducel, Dr. C. Rossi)

Affinity separation

Biosensors Biochips Immunoanalysis

Directed synthesis

and catalysis

AntibodiesEnzymes

Hormon receptors

Highly specific molecular recognition

but

UnstableExpensive

Sometimes difficult to obtain

SYNTHETIC RECEPTORS ?

Drug targeting

Antigen

Monomers Complex Imprinted polymer

MOLECULARLY IMPRINTED POLYMERS -SYNTHETIC ANTIBODIES

CHARACTERISTICS

Tailor-made materials

Physical Stability

Chemical Stability

Storage Endurance

Imprint Memory

Binding Strength

Capacity

Easy integration in industrialfabrication processes

Resistant against mechanical stress, highpressures and temperatures up to 120ºC

Resistant against acids, bases, variousorganic solvents, metal ions

Years at RT without loss of performance

Repeated use without loss of memory

KD in nM - µM range

100 µg/g … 10 mg/g

APPLICATIONS

Antibody / receptor binding site mimics

Immunoassays

Drug development / screening

Sensors

Chemical and Biosensors

Multisensors and Biochips

Tailor-made separation materials

Facilitated synthesis / Catalysis (enzyme mimics)

Slow release matrices

Removal of unwanted molecules

Biomedical, agriculture, food, textile,environment, security…

OUR ACTIVITIES

Material engineering: Morphology (Films, nanoparticles…)Water-compatibilityRecognition properties

Surface engineering: NanostructuresPorosityWetting propertiesOptical properties

Separation: Selective extractionChromatography

Sensitive and selective Biomimetic assaysdetection: Acoustic and optical sensors

BiochipsMEMS

BIOMIMETICS

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ELISA-TYPE IMMUNOASSAY

MIPnanospheres

Conjugate2,4-D

Advantages• No laboratory animals !• Fast and easy• Sensitive• High throughput• Robotisable

Chemiluminescence(H2O2, luminol)

N NH

Tobaccoperoxidase

Detection:

2,4-DO C

Cl

Cl O

Antigen-enzyme conjugate

H

Competitor, g/ml

B/B0, %

20

40

60

80

100

0.01 0.1 1 10 100 1000

1

2

3

4

Competitor

1: 2,4-Dichlorophenoxyacetic acid (2,4-D)2: 4-Chlorophenoxyacetic acid3: Phenoxyacetic acid4: 2,4-D-methylester

X-reactivit

y

100 %10 %2 %<1 %

O COOH

Cl

Cl

2,4-D(Template)

1 2 3 4

Competitorconcentration

96-well plate

384-well plate

BIOPUCES

Deposition of monomer solution with microcantilever array

Imprinted polymer (model system) Trimethylolpropane trimethacrylate 4-Vinylpyridine Porogen : DMSO/Diglyme Antigen : Fluorescein

Photopolymerisation (DPAP, 360 nm)

Vandevelde et al. (2007) Langmuir 23, 6490-6493.

NANOSTRUCTURED SURFACES BY NANOMOULDING

CHANGE OF SURFACE PROPERTIES

Flat surface

Nanostructuredsurface

0 min 1 min 3 min

• Poly(divinylbenzene-co-methacrylic acid) MIP• Environmental scanning electron microscopy• Condensation of water

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Water dropleton lotus leaf

• SAS since 19 July 2004, Rouen, Normandy, France

• Founders : Kaynoush Naraghi Sami Bayoudh Karsten Haupt

• Developing innovative polymers : Molecularly imprinted polymers, responsive hydrogels, associative polymers

• Life and pharmaceutical sciences, food industry, agroindustry

• In 2004, winner of the Contest "Innovative and Technological Companies" of the French Ministry of Research&Technology

• Currently 10 people

• 3rd round of fund raising beginning 2008

VALORISATION